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mRNA-seq

Brief

The research object of eukaryotic mRNA sequencing analysis is the sum of all mRNAs that can be transcribed by a specific cell in a certain functional state. Transcriptome research is the basis and starting point for the study of gene function and structure. Through the new generation of high-throughput sequencing, almost all the transcriptome sequence information of a specific tissue or organ of a species in a certain state can be obtained.

                               
       
Parameters
Product TypeSequencing StrategyIndicatorCycle
mRNA-seqPE150With reference: 6/8/10 G clean data14 days
Without reference: 6/8/10 G clean data24 days
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Requirements
Product TypeSampling Requirements
mRNA-seqRequirement ≥ 0.3 μg
Concentration≥30 ng/μl
PurityOD260/OD280=1.8-2.2
RNA Integrity RIN≥4
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