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10x Genomics Single Cell Transcriptome Sequencing

Brief

10x Genomics is a single-cell library preparation system based on microfluidic technology, which can simultaneously obtain more than 1,000-80,000 single cells, prepare libraries compatible with Illumina platform, classify cell populations and analyze differences in gene expression between cell populations through sequencing of high-throughput single cell data. 

     
Requirements

Sampling Requirements
Sample TypeFresh Samples, Cryopreserved Samples, Fresh Biofluids, Cell&Nuclear Suspension; Samples not suitable for fixation, staining, etc.
Total Amount of SampleTotal amount >6*105 PCS; Duplicate aliquots from one cell sample are processed in parallel: for QC and experimental use.
Sample Concentration700-1200 cells/μL
Sample QualityCells should be free of clumps, with less fragments, survival rate >90%, cell diameter< 30 μm, and free of reverse transcription inhibitors and non-cellular nucleic acid molecules
Cell Cryoprotectant SamplePlease consult local dealers for details
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